primary antibody rat anti-mouse osteocalcin  (Thermo Fisher)

Journal: Bone reports

Article Title: Role of cartilage and bone matrix regulation in early equine osteochondrosis.

doi: 10.1016/j.bonr.2023.101653

Figure Lengend Snippet: Fig. 5. Photomicrographs depicting protein expression for osteocalcin following immunohistochemical localization. A) Osteochondral sample of foal with confirmed OC showing moderate to strong osteocalcin expression in chondrocytes along the osteochondral junction and in within the deeper cartilage layers. B) Negative control for (A) following substitution of mouse monoclonal osteocalcin antibody with nonimmune serum. C) Osteochondral samples of normal foal showing similar results (bar = 100 μm).

Article Snippet: Immunohistochemistry (IHC) was performed on paraffin-embedded osteochondral sections using mouse monoclonal primary antibodies for Osteocalcin (cat#MAB1419, R&D Systems, Inc., Minneapolis, MN) and Collagen type IIB (clone M2139, Thermo Fisher Scientific, Waltham, MA) and rabbit polyclonal primary antibodies for Lubricin (ab28484, Abcam, Cambridge, UK) and SOX9 (PA5-23383, Thermo Fisher Scientific, Waltham, MA).

Techniques: Expressing, Immunohistochemical staining, Negative Control

Journal: Biomaterials

Article Title: The use of heparin/polycation coacervate sustain release system to compare the bone regenerative potentials of 5 BMPs using a critical sized calvarial bone defect model

doi: 10.1016/j.biomaterials.2022.121708

Figure Lengend Snippet: Effects of 5 different BMPs on the osteogenic differentiation of hMDSCs at day 28 (3D pellet culture). A. MicroCT 3D images showed that the mineralized pellets in 5 BMPs groups were larger than the CTL group. Scale bar=1mm. B. Quantification of mineralized pellet volume. All BMPs groups showed higher mineralized pellet volume compared to the CTL group. The mineralized pellet volume of the BMP2 group was significantly higher than the BMP6 and BMP7 groups. *P<0.05, ** P<0.01, ****, P<0.0001. C. Mineralized pellet density quantification. No statistical difference between BMP groups and the CTL group was found. D. Von Kossa staining. Brown-black color indicates mineralization. All pellets have regions peeled off due to high mineralization in the white region of pellets. E. Immunohistochemistry of osteocalcin for osteogenic pellets. Brown color indicates osteocalcin expression. The mineralized parts of the pellets demonstrate crystal purple color intermingled with osteocalcin brown staining. Scale bars=100μm.

Article Snippet: Osteocalcin immunohistochemistry using a mouse anti-human osteocalcin primary antibody (MAB1419, 1:100, R&D Systems) was also performed, as previously described [ 27 ].

Techniques: Staining, Immunohistochemistry, Expressing

Journal: Tissue Engineering. Part C, Methods

Article Title: Development of Modular, Dual-Perfused Osteochondral Constructs for Cartilage Repair

doi: 10.1089/ten.tec.2018.0356

Figure Lengend Snippet: Osteocalcin expression in osteogenic microbeads. (A) IHC revealed osteocalcin (brown) within osteogenic microbeads cultured under both osteogenic and control conditions (scale bar = 100 μm). (B) Osteocalcin was detected through ELISA in conditioned medium of control (agarose only) microbeads cultured under osteogenic conditions (*p ≤ 0.05 compared with other groups). ELISA, enzyme-linked immunosorbent assay; IHC, immunohistochemistry. Color images are available online.

Article Snippet: Next, sections were blocked and subsequently incubated overnight at 4°C in a mouse anti-rat osteocalcin primary antibody (R&D Systems) in tris-buffered saline (TBS).

Techniques: Expressing, Cell Culture, Control, Enzyme-linked Immunosorbent Assay, Immunohistochemistry

Journal: Tissue Engineering. Part C, Methods

Article Title: Development of Modular, Dual-Perfused Osteochondral Constructs for Cartilage Repair

doi: 10.1089/ten.tec.2018.0356

Figure Lengend Snippet: Osteogenic differentiation in constructs as indicated by osteocalcin IHC (brown staining). Dotted lines mark the physical interface between phases (scale bar = 100 μm). Color images are available online.

Article Snippet: Next, sections were blocked and subsequently incubated overnight at 4°C in a mouse anti-rat osteocalcin primary antibody (R&D Systems) in tris-buffered saline (TBS).

Techniques: Construct, Staining